Please join us on Tuesday, May 14th, 2019 at Northwestern, Ryan 4003. Dinner starts at 6:30 PM with the seminar immediately following at 7:00 PM. Please be sure to register so our sponsors know how much food to order! We hope you can join us! To register click here

Sponsored by

Speaker: Charlie Yang, Research Assistant Professor, Rosalind Franklin University of Medicine and Science

Title:Quantitative Proteomics by Mass Spectrometry
— Applications and Challenges

Abstract: Mass Spectrometry based quantitative proteomics technique is easy to try but hard to execute
because multiple factors have to be considered depending on what strategy you would use, what
specific approaches you choose, and what instruments you perform to conduct your research. This topic
will profile the comprehensive views for quantitative proteomics by mass spectrometry, mainly focus on
the current status of relative and absolute quantitation of proteins. The primary emphasis is on the
combination of liquid chromatography coupled with mass spectrometry (LC-MS) and associated tandem
mass spectrometry (LC-MS/MS) techniques, which involves a secondary analysis within the instrument
for peptide level and protein level for determination and quantitation. Furthermore, the major
approaches including label-free and isotope-labeling for relative and absolute quantitation will be
focused to make quantitative measurement of protein expression. Then a discussion follows on the
various computational tools used to quantify peptides and proteins from LC-MS/MS data.