Please join us on Tuesday, March 7th, 2017 for a seminar at the University of Illinois at Chicago, College of Pharmacy 212/216 833 S. Wood St, Chicago, IL 60612, by Vicki Wysocki from the Ohio State University. Dinner, sponsored by Thermo, will be at 6:00 with the seminar to start at 6:30 pm. ** Note the Change in TIME **
Title: Native MS in Structural Biology: Characterization of Protein and Nucleoprotein Complexes by SID/IMMS or SID/HRMS
Abstract: Characterization of the overall topology and inter-subunit contacts of protein complexes, and their assembly/disassembly and unfolding pathways, is critical because protein complexes regulate key biological processes, including processes important in understanding and controlling disease. Conventional structural biology methods such as X-ray crystallography and nuclear magnetic resonance provide high-resolution information on the structures of protein complexes. However, other emerging biophysical methods that provide lower resolution structural data (e.g. stoichiometry and subunit connectivity) on the structures of the protein complexes are also important. Native mass spectrometry is an approach that provides critical structural information with higher throughput on low sample amounts. The power of native MS increases when coupled to ion mobility (IM-MS), a technique that measures rotationally averaged collisional cross sections and thus direct information on conformational changes, or to high resolution mass spectrometry (HRMS). This presentation illustrates surface-induced dissociation/ion mobility SID/IM MS and SID HRMS for characterization of topology, intersubunit connectivity, and other structural features of multimeric protein complexes. Data for a number of protein-partner complexes are under investigation, where the partner can be small molecule ligand, protein, DNA, or RNA.
About Dr. Wysocki:
Vicki Wysocki received her PhD from Purdue University in 1987, under the direction of Professor Graham Cooks. Following a National Research Council postdoctoral appointment at the US Naval Research Laboratory, she became an Assistant Professor at Virginia Commonwealth University. She joined the University of Arizona in 1996 and eventually served as Chair of the Department of Chemistry and Biochemistry. In 2012 she moved to The Ohio State University where she is Ohio Eminent Scholar of Macromolecular Structure and Function and Director of the Campus Chemical Instrument Center. In 2009, she received the Distinguished Contribution to Mass Spectrometry Award from the American Society for Mass Spectrometry, jointly with Professor Simon Gaskell. She is the 2017 ACS Field and Franklin Awardee for Outstanding Contributions to Mass Spectrometry. Her laboratory is part of the Waters Center of Innovation program. She is an associate editor for the ACS journal Analytical Chemistry. She has completed two years as Vice President for Programs of the American Society for Mass Spectrometry and assumed a two-year tenure as ASMS President in July, 2016.
Professor Wysocki’s research interests include bioanalytical mass spectrometry, peptide fragmentation mechanisms, proteomics and metabolomics (Invasive Aspergillosis, non-small cell lung cancer, pre-eclampsia; Salmonella), ion-surface interaction chemistry, and instrument development for improved dissociation and characterization of non-covalent protein complexes.
About the Site:
The College of Pharmacy Building can be found at 833 S. Wood Street, Chicago, IL 60612. If driving, please park in either the Wood Street Parking Structure (1100 S. Wood St) or the Paulina Street Parking Structure (915 S. Paulina). If coming by CTA, take the Pink line to the Polk stop.
Please be sure to register in advance so our sponsors know how much food to order! We hope you can join us!